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HPLC determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde
Summary
Type:
HPLC
Application #:
107770
Catalog#:
720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection:
fluorescence, excitation 330nm, emission 418nm
Conditions:
eluent A: 0.1M phosphate buffer pH 7.5, eluent B: methanol, linear gradient from 40 to 75% B in 23min
flow rate 1ml/min
Authors:
Lindroth, P. et al.
Source:
Anal. Chem. 51 (1979) 1667–1674
Details
Legend: Fig. 8. Protein hydrolyzate, 2. Asp, 3. Glu, 6. Ser, 8. His, 10. Thr, 11. Gly, 12. Arg, 15. Ala, 19. Val, 20. Phe, 21. NH4+, 22. Ile, 23. Leu, 25. Lys.
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